Coding
Part:BBa_K4968002:Design
Designed by: Shouye Zhu Group: iGEM23_XJTLU-CHINA (2023-10-09)
Optimized CBM
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 90
Illegal BsaI.rc site found at 83
Design Notes
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Source
The sequence of the family 2 CBM from endoglucanase A of C.fimi was obtained from NCBI.